RNA-seq transcriptomics

Quantification of gene expression levels

The transcriptome is the set of RNA molecules present in a cellular environment at a given point in time. Most of this RNA originates from the transcription of active genes under specific environmental conditions.

Total RNA-seq transcriptomics quantifies the RNA present in a sample, including messenger RNA, non-coding RNA, and precursor RNA depending on library preparation: poly-A selection (eukaryotes) or ribodepletion (prokaryotes/total RNA). The result is a high-resolution gene expression profile, which we use to perform differential analysis (genes that change between conditions) and enrichment analysis, delivering robust metrics (log2FC, FDR) and interpretable visualisations.

As a complement to microbial community studies, total RNA-seq (or its community-level equivalent, metatranscriptomics) provides the active functional layer whereas metagenomics describes genetic potential (which genes are present), RNA-seq reveals which genes are being expressed and at what level under a specific context (treatments, time, stress). Integrating metagenomics and RNA-seq allows taxonomic profiles to be linked to real functional activity, enabling prioritisation of targets and a deeper understanding of biological mechanisms.

We at Microomics Systems offer a total RNA-seq service tailored to academic and industrial projects that require gene expression quantification in areas such as cell biology, product evaluation, pharmaceuticals, treatments, management practices, human clinical research, and other sectors.