Animal Health

 

 

 

Human Health

 

 

 

Agriculture

 

 

 

Food Industry

 

 

 

USES

  • Sequencing and analysis of genomes of pathogens and probiotics.

  • Effects of the application of additives and/or pre/probiotics on the microbiome.

  • Study of the microbiome in clinical and field trials with a focus on health, nutrition, and reproduction.

  • Characterization of resistance to antibiotics/antimicrobials.

  • Study of gene expression in in vitro and in vivo assays.

  • Detection and quantification of pathogens by qPCR.

  • Detection and monitoring of microorganisms in probiotics by qPCR.

  • Analysis on multiple matrices: feces, intestinal content, semen, vaginal samples, etc.

 

USES

  • Sequencing and analysis of genomes of pathogens and probiotics.

  • Impact of the microbiome on infectious diseases and resistance to treatment.

  • Involvement of the microbiome in intestinal, metabolic, vaginal and gut-brain axis diseases.

  • Study of the microbiome in clinical trials with a focus on health and nutrition.

  • Study of the microbiome in pre-clinical trials in animal models.

  • Effects of the use of pre/probiotics on the microbiome.

  • Study of gene expression in in vitro and in vivo assays.

  • Analysis on multiple matrices: feces, skin, saliva, nasal swabs, vaginal samples, etc.

USES

  • Environmental studies: water, soil, plantations.

  • Sequencing and analysis of pathogen genomes and plant growth promoters.

  • Study of the microbiome of the soil or crops against the application of fertilizers, biostimulants, fungicides, herbicides, etc.

  • Detection and quantification of pathogenic microorganisms in cultures by qPCR.

  • Study of gene expression in in vitro and in vivo assays.

  • Analysis on multiple matrices: soil, plant material, water, etc.

  • Field trials or production samples.

USES

  • Study of the microbiome in fermented products

  • Detection and monitoring of pathogens/contaminants using qPCR.

  • Sequencing and analysis of genomes of fermenting microorganisms or of biotechnological interest.

  • Identification of food components through barcoding.

Barcoding
Metagenomics

Analysis of the taxonomic profile and diversity of the microbiota through the amplification and sequencing of the variable regions V3 and V4 of the 16S ribosomal RNA gene (16S rRNA) using Illumina MiSeq (300×2).

The service includes:

  • DNA extraction, library preparation and sequencing.
  • Bioinformatic analysis:
    • Diversity and taxonomic profiles based on ASVs.
    • Statistical inference.
    • Figures and graphs available for publication.
  • Personalized report delivery and post-delivery monitoring.

Possibility of using other markers (ITS, 18S, COI).

Analysis of the taxonomic and functional profile of the microbiota by sequencing the total DNA of the sample using Illumina NovaSeq 6000 (100×2 / 150×2).

The service includes:

  • DNA extraction, library preparation and sequencing.
  • Bioinformatic analysis:
    • Assembly of metagenomes.
    • Functional and taxonomic annotation.
    • Statistical inference (comparative metagenomics).
    • Figures and graphs ready for publication. 
  • Personalized report delivery and post-delivery monitoring.

Possibility of extending the functional annotation with specific databases (pathogenicity, antibiotic resistance, virulence, bioremediation, etc.).

Shotgun
Metagenomics

Transcriptomics
(RNASeq)

Analysis of the differential expression between groups or experimental conditions. We used the RNAseq approach using the Illumina NovaSeq 6000 (100×2 / 150×2).

The service includes:

  • mRNA extraction, library preparation and sequencing.
  • Bioinformatic analysis:
    • Alignment against reference genome.
    • Statistical inference (using DESeq2).
    • Figures and graphs ready for publication.
  • Personalized report delivery and post-delivery monitoring.

Sequencing and annotation of genomes of isolated microorganisms using Illumina NovaSeq 6000.

The service includes:

  • Quality control of the isolate with Sanger Sequencing of the 16S RNA gene.
  • DNA extraction, library preparation and sequencing.
  • Bioinformatic analysis:
    •  Assembly.
    • Annotation.
  • Personalized report delivery and post-delivery follow-up.

Possibility of personalizing the analysis and annotation of the genome with specific databases (pathogenicity, resistance, virulence, bacteriophages, etc.) and prospecting for genes or enzymes of interest.

Prokaryotic
Genomics

qPCR/PCR

Amplification of certain sequences of genetic material:

  • PCR: DNA amplification.
  • qPCR: quantitative DNA amplification.
  • RT-PCT: RNA amplification (Reverse Transcrption-PCR).

FROM BEGINNING TO END

We offer a 360º service, providing support at all stages of the service.

  • Initial advice: for the development and optimization of the experimental design. We include recommendations and protocols for the collection, conservation and shipment of samples.
  • Wet Lab processing: DNA extraction, library preparation and sequencing. We maintain active communication to confirm the results of quality controls at each stage. We have experience in the processing of multiple matrices, including those samples that present the greatest difficulty (biopsies, breast milk, etc.).
  • Bioinformatic and biostatistical analysis: sequencing data processing and statistical analysis adjusted to the experimental design. We also offer the commissioning service for specific pipelines on demand.
  • Final report: we deliver the results in a dynamic and interactive format. We hold meetings to discuss methods and results. We guarantee post-delivery tracking.

Microomics’ goal is to guarantee the highest quality at all stages of our analysis, to offer high-quality services that add value to your projects.

EXPERIENCE AND QUALITY

The Microomics team is made up of specialized professionals with different scientific profiles, such as microbiology, bioinformatics, mathematics and genetics, among others. This diversity allows us to provide high quality services, and also act as consultants and experts in the validation of results, providing individualized and specific support through the creation of customized solutions.

We have participated in more than 500 national and international projects, in more than 12 countries and with more than 130 entities. Our clients are from the academic, public and private sectors as well as production sectors in the field of human and animal health and nutrition, and the agro-environmental field and the consumer goods industry.

Ask us about a service

So that we can provide you with more information and a personalized offer, tell us what service you are interested in and the approximate number of samples to be analysed.


     

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